rabbit anti col1 antibodies  (Affinity Biosciences)

Journal: Communications Biology

Article Title: GDF11 alleviates glucocorticoid-induced osteonecrosis of the femoral head by regulating angiogenesis via the PI3K-AKT-eNOS pathway

doi: 10.1038/s42003-025-09078-5

Figure Lengend Snippet: A , B Angiography of femoral heads and subsequent vessel volume quantification in the experimental groups: sham, MP, MP + AAV-NC, MP + AAV-GDF11 (n = 6 per group). C Representative transverse sections of H&E and Masson staining from the sham, MP, MP + AAV-NC, and MP + AAV-GDF11 groups are shown. Scale bar = 500 μm. D Quantification of angiography particles number in different groups (n = 6 per group). E Representative immunofluorescence images of the sham, MP, MP + AAV-NC, and MP + AAV-GDF11 groups showing CD31 (green), COL1 (red), and DAPI-stained nuclei (blue). Scale bar = 100 μm. F , G Quantitative analysis of mean fluorescence in different groups (n = 6 per group). H , I IHC staining for CD31 and quantitative analysis of CD31-positive cells in the sham, MP, MP + AAV-NC, and MP + AAV-GDF11 groups (n = 6 per group). Scale bar = 100 μm (upper panel) and 50 μm (lower panel). Significant differences are indicated as follows: ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001.

Article Snippet: Primary antibodies like COL1 (#72026, Cell Signaling Technology), VEGFA (ab52917, Abcam), CD31 (ab182981, Abcam), p-PI3K (TA3242, Abmart), p-eNOS (PC3447, Abmart) and corresponding fluorescent secondary antibodies (ab150079 and ab150077, Abcam) were used.

Techniques: Staining, Immunofluorescence, Fluorescence, Immunohistochemistry

Journal: Bone & Joint Research

Article Title: High-intensity running exercise promotes knee meniscal damage via the PI3K/AKT/mTOR axis

doi: 10.1302/2046-3758.1411.BJR-2024-0535.R1

Figure Lengend Snippet: Excessive mechanical stress promotes meniscus degeneration.a) Immunohistochemical staining revealed the expression of Aggrecan and matrix metalloproteinase 13 (MMP13) in the anterior horn, posterior horn, and articular surface of the meniscus following treadmill intervention in mice. Scale bar: 100 µm. (The same group showed serial sections from the same mouse knee joint tissue for staining analysis.) b) Statistical analysis of the differences in MMP13 and Aggrecan expression between the anterior and posterior horns of the meniscus and the articular cartilage surface groups (n = 5/group). Data were analyzed using one-way analysis of variance (ANOVA) followed by Tukey's post-hoc test. c) Immunofluorescence displaying COL1-positive, Aggrecan-positive, MMP13-positive, and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5)-positive cells as a proportion of total meniscal cells in primary medial meniscal cells (n = 3/group). Data are presented as the mean (SD). Scale bar: 200 µm. d) Quantification of the positive meniscus cells (P-PI3K, P-AKT, and P-S6) as a proportion of total meniscal cells (n = 3/group). Data were analyzed using independent-samples t -test. *p < 0.05, **p < 0.01, ***p < 0.001. ns, non-significant.

Article Snippet: Mouse anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1:10,000, ab8245; Abcam, UK), rabbit anti-MMP13 (1:2,000, 18165-1-AP; Proteintech, USA), rabbit anti-A disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5) (1:1,000, A2836; Abclonal, USA), rabbit anti-collagen type I (COL1) (1:2,000, 14695-1-AP; Proteintech), rabbit anti-Aggrecan (1:1,000, A11691; Abclonal), rabbit anti-phospho-PI3K p85 (1:1,000, YP0224; ImmunoWay, USA), rabbit anti-phospho-AKT (Thr308) (1:500, PC2720; Abmart, China), rabbit anti-phospho-S6 (S240/S244) (1:500, AP0537; Abclonal), rabbit anti-PI3K (1:500, A17433; Abclonal), rabbit anti-AKT (1:1,000, ab192623; Abcam), and rabbit anti-S6 (1:500, A6058; Abclonal) served as the primary antibodies used.

Techniques: Immunohistochemical staining, Staining, Expressing, Immunofluorescence